ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of human telomerase catalytic subunit, hTERT, in human non-small cell lung cancer (NSCLC) and its correlations to c-myc gene.</p><p><b>METHODS</b>hTERT and c-myc mRNA expressions were detected by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). Statistical correlation analysis was made to estimate whether there was interrelation between them.</p><p><b>RESULTS</b>Positive rate of hTERT expression in 51 surgically resected lung cancer specimens was 86.3%, significantly higher than that in adjacent non-neoplastic lung tissues and benign lesions, which were 14.3% and 27.3% respectively. No statistical significance was observed between the frequency of hTERT expression and histologic types, degree of differentiation, TNM stages, tumor size or lymph nodes metastases. Correlation analysis revealed that the expression of c-myc gene was significantly related to that of hTERT (correlation coefficient, r = 0.633, P < 0.001).</p><p><b>CONCLUSIONS</b>hTERT may be a useful tumor marker in diagnosing lung cancer. Significant correlation between the expression of hTERT and c-myc mRNA indicates that the activation and up-regulation of hTERT might be conferred by over-expression of c-myc gene.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Adenocarcinoma , Genetics , Pathology , Biomarkers, Tumor , Carcinoma, Adenosquamous , Genetics , Pathology , Carcinoma, Non-Small-Cell Lung , Genetics , Pathology , Carcinoma, Squamous Cell , Genetics , Pathology , DNA-Binding Proteins , Genes, myc , Genetics , Lung Neoplasms , Genetics , Pathology , Reverse Transcriptase Polymerase Chain Reaction , TelomeraseABSTRACT
<p><b>BACKGROUND</b>To detect the expression of human epidermal-growth-factor receptor 4 (HER4) and elucidate the relationship between its overexpression and the clinicopathological characteristics in non-small cell lung cancer (NSCLC).</p><p><b>METHODS</b>The expression of HER4 was detected in 70 cases of paraffin-embedded NSCLC tissues by immunohistochemical assay.</p><p><b>RESULTS</b>HER4 were overexpressed in 91.4% of NSCLC. The overexpression of HER4 was significantly related to lymph node metastasis (P=0.007), TNM stages (P=0.011) and postoperative survival rate (P= 0.0258).</p><p><b>CONCLUSIONS</b>erbB4 is one of the genes to regulate the growth of advanced NSCLC. The artificial interference with HER4 overexpression may be a good way in the treatment of advanced NSCLC.</p>
ABSTRACT
Objective To study the etiolgy and clinical features of pathological fracture in femur. Methods 17 cases of pathological fracture in femur,including 10 males and 7 females with average age 39.5(13～74),were all treated surgically and their pathological diagnosis were achieved,which were consistent with the clinical diagnosis.Results 12 patients (70.59%) suffered from fracture resulted from malignant tumor,of which lung cancer and breast cancer were the commonest with 3 cases respectively (50%).The fracture of proximal end accounted for 65%.8 cases of spontaneous fracture and 2 cases of multiple fracture were all malignancy sufferers.Conclusion Malignancy is the main cause of pathological fracture in femur,of which lung cancer and breast cancer are the commonest.Proximal end of femur is the most frequent part to which fracture happens.Both spontaneous and multiple fractures are resulted from malignancy.